|
| Primer name | Primer sequence | Size (bp) and location |
|
| APO-F1 |
5′ CCSTGCTGTTGGTCMCATT 3′ | Degenerate primers (nest primer) |
| APO-R1 | 5′ GAGGCCTGTATCTTCTCC 3′ | 600 bp |
| APO-F2 | 5′ GAGCYGGAGGTGACAGAT 3′ | |
| APO-R2 | 5′ GGCGMTGCATGTCTTCCACTA 3′ | |
| APO-E-R1 | 5′ GCT CCT TTG TGT AAG CCT TCA C 3′ | 5′RACEprimer (nest primer) |
| APO-E-R2 | 5′ ATG GTG TCC TCT ATC AGA ACC GTC 3′ | 400 bp |
| APO-E-F1 | 5′ CAA GAT GGA GGA GCA GAC ACA 3′ | 3′RACEprimer (nest primer) |
| APO-E-F2 | 5′ GGA GGA GCA GAC ACA GCA GAT 3′ | 300 bp |
| APO-intron-F1 | 5′ ATGAAGGCTCTGTGGGCTG 3′ | Fish for ApoE introns |
| APO-intron-R1 | 5′ AAGCCTTCACTTCTGTCATGGTGT 3′ | (nest primer) 195 bp |
| APO-intron-F2 | 5′ GTCGCGTTGTTGGCAGGAT 3′ | |
| APO-intron-R2 | 5′ GTCCTCTATCAGAACCGTCAG 3′ | |
| APO-intron-F3 | 5′ TGCAGACGCTGTCTGACCA 3′ | Fish for ApoE introns |
| APO-intron-R3 | 5′ CCAGCATGGTCTGTACCTC 3′ | (nest primer) 169 bp |
| APO-intron-F4 | 5′ GACGGTTCTGATAGAGGACAC 3′ | |
| APO-intron-R4 | 5′ GCCCAGTCTGTTGCGAAG 3′ | |
| APO-intron-F5 | 5′ GACATGGAAGACCTTCGCAACA 3′ | Fish for ApoE introns |
| APO-intron-R5 | 5′ CCTGGTTGCCCATCAGCT 3′ | (nest primer) 287 bp |
| APO-intron-F6 | 5′ GCAGCGAGGTACAGACCA 3′ | |
| APO-intron-R6 | 5′ GATGCGGGCACCCAAAGC 3′ | |
| APO-intron-F7 | 5′ TGGTGGAGGAAGGTCGCC 3′ | Fish for ApoE introns |
| APO-intron-R7 | 5′ GTAGGGAGGATGGGATTGGT 3′ | (nest primer) 227 bp |
| APO-intron-F8 | 5′ GTCGGCTGGAGCTGATGG 3′ | |
| APO-intron-R8 | 5′ GGGATTGGTAGCCACGGA 3′ | |
| APO-Full-F1 | 5′ CCC GAA GGC TAA GGT TTT G 3′ | Contig primer |
| APO-Full-R1 | 5′ ACC TGC TGG TCG TGG AT 3′ | (Nest primer) 1800 bp |
| APO-Full-F2 | 5′ GGC TGG TTC ATC ACA GTT GTG 3′ | |
| APO-Full-R2 | 5′ TGG AGA GGG ATC TTC ATT GAC TCT 3′ | |
| liuF1 | 5′ GGTCGCGTTGTTGGCAGGTA 3′ | SSCP primer, 410 bp |
| liuR1 | 5′ ATAGGACAGAGCTGAGACCA 3′ | |
| liuF2 | 5′ AGACGCTGTCTGACCAGGTC 3′ | SSCP primer, 449 bp |
| liuR2 | 5′ AGGGCCTAGACAGAAGGGCC 3′ | |
| liuF3 | 5′ CTGGAGCTGATGGGCAACCA 3′ | SSCP primer, 275 bp |
| liuR3 | 5′ GGGGTGGAGAGGGATCTTCA 3′ | |
| liuF4 | 5′ CCCCCGAAGGCTAAGGTTTT 3′ | SSCP primer, 225 bp |
| liuR4 | 5′ ATAAAAAAGCTGCTCGGGGC 3′ | |
| liuF5 | 5′ CTGGAGCTGAT GGGCAACCA 3′ | SSCP primer, 347 bp |
| liuR5 | 5′ AAGTAAGGGCCACCAGAGGG 3′ | |
|
