Ex Vivo Expansion of Functional Human UCB-HSCs/HPCs by Coculture with AFT024-hkirre Cells

Figure 2

Shedding of hkirre extracellular motif in supernatants and its supportive activity. (a) 1×10⁶/mL AFT024-hkirre cells, (A) and control cells (B) were cultured for 48 hours and supernatants were collected. A protein of roughly 27 kDa was detected by SDS-PAGE stained with commassive brilliant blue. (b) 1×10⁵ CD34⁺ cells/well in 24-well culture plates were cultured with supernatants of AFT024-hKirre cells or AFT024 control cells. All cells were harvested from wells by gentle pipetting and counted with hemocytometer. For flow cytometry analysis, cells were stained with PE-conjugated mAb to CD34 and FITC-conjugated mAb to CD38. Values indicate the fold increase compared with the initial number of cells. The results were expressed as mean ± SD (𝑛=4). ^∗𝑃<0.05 and ^∗∗𝑃<0.01 compared between SN of AFT024-hkirre and AFT024 control cells (Student’s 𝑡-test).