Ex Vivo Expansion of Functional Human UCB-HSCs/HPCs by Coculture with AFT024-hkirre Cells

Figure 4

The effect of the AFT024-hKirre cells on LTC-IC assay of CD34⁺ cells. 1.0×10⁴ CD34⁺ CB cells were cocultured with AFT024-hkirre cells or AFT024 control cells for 5–8 weeks and then subjected to CFU assay. After 16 days of culture, the colonies, including BFU-Es, CFU-GMs, and CFU-Mix’s, with greater than 50 cells were counted. The results are expressed as mean ± SD (𝑛=3). ^∗𝑃<0.05 and ^∗∗𝑃<0.01 compared between AFT024-hkirre and AFT024 control cells (Student’s 𝑡-test).

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