Ex Vivo Expansion of Functional Human UCB-HSCs/HPCs by Coculture with AFT024-hkirre Cells

Figure 5

Effect of AFT024-hkiree cells on the reconstitution ability of CD34⁺ cells in NOD/SCID mice. UCB-HSCs were coculture with AFT024-hkirre or control cells and harvested after 4 week. 3×10³ cells were injected intravenously into sublethal irradiated NOD/SCID mice. For positive control, freshly isolated CD34⁺ cells, and for negative control, only medium was injected. Each group comprises of three mice at least. After 8 weeks of transplantation, mice were sacrificed,and mononuclear cells were harvested and analyzed for human CD45⁺ cells by flow cytometry ((a) graph showing the percentage of hCD45⁺ cells and (b) for the expression of human specific 17𝛼-satellite gene by PCR). The results are expressed as mean ± SD (𝑛=4). ^∗𝑃<0.05 and ^∗∗𝑃<0.01 compared between AFT024-hkirre and AFT024 and AFT024-hkirre and freshly isolated CD34⁺ cells (Student’s 𝑡-test).

(a)

(b)