Ex Vivo Expansion of Functional Human UCB-HSCs/HPCs by Coculture with AFT024-hkirre Cells
Figure 6
Genes expression profile of AFT024 cells interacted with CD34+ cells and semiquantitative RT-PCR of selected genes. The figure shows the effect of interaction between hUCB-HSCs/HPCs and AFT024-hkirre cells on the transcription of Wnt-5A, BMP4, SDF-α, and TGF-. The AFT024-hkire cells () were cocultured with CD34+ cells (/well) in six-well plates for 24 hr and then washed twice to remove loosely adherent and nonadherent cells. The adherent cells were harvested and analyzed by semiquantitative RT-PCR for transcripts of the above-mentioned genes. Fold changes at 35 cycles were determined through densitometric analysis using Fluor-STM Multi-imager and Quality One 4.3.0 software (Bio-RAD) (a) expression from AFT024-hkirre cells, (b) expression from the AFT024-control cells, (c) folds of change from AFT024-hkirre group, and (d) folds of changes from control group. The data shown was a representative experiment of three reproducible experiments. (e) Cytokine (profile) expression profile was analyzed by RT-PCR. Upper panels show AFT024-hKirre cells group, and lower panels show the AFT024 control cells group.
