Ex Vivo Expansion of Functional Human UCB-HSCs/HPCs by Coculture with AFT024-hkirre Cells

Table 2

List of primers used in this study.

Gene	Primers	Product size (bp)
TPO	5′-TTC CAA CAC CTG CTC CG-3′	334
	5′-CCT TGT GGA GTG CGT GAA-3′
SCF	5′-CAG GAA AGA GTC CAC GAG TT-3′	323
	5′-TGG CTG CCC AGT GTA GG-3′
Beta-Actin	5′-AGCGGGAAATCATGCGT-3′	515
	5′-CTA GAA GCA TTT GCG GTG G-3′
FLt3	5-CTGGAGCCCAACAACCTATC-3′	181
	5′-GGA CGG TGA CTG GGT AAT CT-3′
MMP9	5′-TCCCTGGAGACCTGAGAACC-3′	519
	5′-AATGGGCGTCTCCCTGAAT-3′
Jagged-1	5-GAT CCT GTC CAT GCA GAA CG-3	440
	5-GGA TCT GAT ACT CAA AGT GG-3
Wnt-5A	5-ACA CCT CTT TCC AAA CAG GCC-3	341
	5-GGA ATT GTT AAA CTC AAC TCT C-3
HOXB4	5′-TCA CAG AGC GAT TAC CTA CCC A-3′	498
	5′-CCG TGT CAG GTA GCG GTT GT-3′
Delta-like 1	5′-AGA CGG AGA CCA TGA ACA ACC-3′	341
	5′-TGA AGT TGA ACA GCC CGA GT-3′
Angio 1	5′-AAT AAT ATG CCA GAA CCC-3′	500
	5′-AGA TAG TCA ACC TAC GAA A-3′
TGF-b	5′-CAA GTG GAC ATC AAC GGG TT-3	297
	5′-GCT CCA AAT GTA GGG GCA GG-3′
SDF alpha	5′-CAC TCC AAA CTG TGC CCT TCA-3′	200
	5′-TCC TTT TCT GGG CAG CCT T-3′
Shh	5′-ACT GGG TGT ACT ACG AGT CCA AGG-3′	211
	5′-AAA GTG AGG AAG TCG CTG TAG AGC-3′
17 𝛼-satellite	5′-ACG GGA TAA CTG CAC CTA AC-3′	234
	5′-CCA TAG GAG GGT TCA ACT CT-3′
LIF	5′-GCC AAC GGC ACG GAG AA-3′	128
	5′-TTG CTG TGG AGG CTG AGG G-3
BMP-4	5′-CAGCAGCATCCCTGAGAACGAG-3′	306
	5′-GTCCGAGTCTGATGGAGGTGAG-3′
VEGF	5′-TCG GGC CTC CGA AAC CAT GA-3′	649
	5′-CCT GGT GAG AGA TCT GGT TC-3′