Research Article
A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus
Table 1
Yields and laccase activities of various chromatographic fractions (from 50 g mycelia).
| | Fraction | Yield (mg) | Total activity (U) | Specific activity (U/mg) | Recovery of activity (%) | Purification fold |
| | 80% ammonium sulphate fractionation | 996 | 13814.42 | 13.86 | 100 | 1 | | D1 | 104.18 | — | — | — | — | | D2 | 85.89 | — | — | — | — | | D3 | 35.31 | 3357.96 | 95.09 | 24.3 | 6.9 | | D4 | 436.16 | — | — | — | — | | C1 | 20.62 | — | — | — | — | | C2 | 14.67 | — | — | — | — | | C3 | 3.234 | 2091.72 | 646.79 | 15.1 | 46.6 | | C4 | 2.28 | — | — | — | — | | Q1 | 1.76 | — | — | — | — | | Q2 | 1.85 | 1334.11 | 721.14 | 9.7 | 52 | | SU1 | 1.48 | 1078.97 | 729.04 | 7.8 | 52.6 | | SU2 | 0.35 | — | — | — | — |
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—: no laccase activity observed. Laccase-enriched fractions were highlighted in boldface.
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