Gold Nanoparticles Inhibit VEGF165-Induced Migration and Tube Formation of Endothelial Cells via the Akt Pathway
Figure 2
The effects of AuNPs on VEGF165-induced HUVEC migration. (a) Confluent HUVEC monolayers were wounded with pipette tips (upper panel) and treated with VEGF165 (20 ng/mL) in the presence or absence of AuNPs (125 or 250 nM). After 24 h incubation (lower panel), the wound closures were imaged to analyze the abilities of the cells to migrate into and fill the wounded areas. HUVECs incubated in 0.5% serum were used as controls. HUVECs treated with VEGF165 migrated efficiently from the wound margin relative to the controls. AuNPs significantly inhibited VEGF165-induced cell migration. (b) The average migration of cells treated with 0.5% serum (control) and VEGF165 (20 ng/mL) in the presence or absence of AuNPs (125 or 250 nM). Data are presented as the means ± SEM based on three independent experiments. compared with the control group; compared with the VEGF165 group.
