Gold Nanoparticles Inhibit VEGF165-Induced Migration and Tube Formation of Endothelial Cells via the Akt Pathway
Figure 3
The effects of AuNPs on HUVEC migration in a Transwell assay. (a) Sample Transwell assay images showing the inhibitory effects of AuNPs on VEGF165-induced HUVEC migration after staining the transmigrated cells with crystal violet (magnification, 10x). VEGF165-treated (20 ng/mL) cells transmigrated through the Matrigel-coated Boyden chamber within 12 h, compared with controls, whereas pretreatment with AuNPs (125 or 250 nM) significantly reduced cell migration. (b) Quantification of the Transwell assay results: crystal violet OD values represent the amounts of transmigrated cells. Data are presented as the means ± SEM based on three independent experiments. compared with the control group; compared with the VEGF165 group.
