Gold Nanoparticles Inhibit VEGF165-Induced Migration and Tube Formation of Endothelial Cells via the Akt Pathway
Figure 7
AuNPs inhibit VEGF165-induced HUVEC migration mediated by Akt pathway. (a) Serum-starved HUVECs were treated with VEGF165 (20 ng/mL) in the presence or absence of gold nanoparticles. The effects of AuNPs on phosphorylated Akt and pan Akt were determined using Western blot analysis. (b) Confluent HUVEC monolayers were wounded with pipette tips (upper panel) and treated with VEGF165 (20 ng/mL) in the Ly294002 (10 M). After 24 h incubation (lower panel), the wound closures were imaged to analyze the abilities of the cells to migrate into and fill the wounded areas. HUVECs incubated in 0.5% serum were used as controls. (c) The average migration of cells treated with 0.5% serum (control) and VEGF165 (20 ng/mL) in the Ly294002 (10 M). Data are presented as the means ± SEM based on three independent experiments. compared with the control group; compared with the VEGF165 group.
