Systemic Approach to Identify Serum microRNAs as Potential Biomarkers for Acute Myocardial Infarction
Figure 1
Detection of differentially expressed circulating miRNAs in serum samples. (a) Detection of miRNAs in serum from healthy subjects. Expression levels of 270 miRNAs in 300 μL of serum were quantified using a multiplexed RT-qPCR assay. The relative expression levels of the top 50 miRNAs detected in serum from three healthy subjects (mean ± SD) are shown. (b) Principle component analysis using expression levels of the 270 human miRNAs in serum samples from 8 healthy (blue) and 8 AMI (red) subjects. (c) Volcano plot indicated that 25 miRNAs were significantly altered in sera from the AMI patients. Red lines indicate the Student’s -test and ±2-fold change. (d) Unsupervised hierarchical clustering of healthy and AMI samples using the 25 differentially expressed serum miRNAs. The hierarchical clustering was generated using Pearson’s dissimilarity as the distance measure and Ward’s method for linkage analysis. A: AMI patients; N: healthy subjects.
