Equine Arteritis Virus Does Not Induce Interferon Production in Equine Endothelial Cells: Identification of Nonstructural Protein 1 as a Main Interferon Antagonist
Figure 2
EAV nonstructural proteins (nsps) involved in suppression of IFN-promoter activation. (a) Expression of nsps in transfected cells detected by indirect immunofluorescence assay. HEK293T cells were transfected with each EAV nsp and fixed at 24 h after transfection. Cells were stained with EAV nsp1 MAb, EAV nonstructural protein-specific rabbit antisera (nsp2, nsp3, nsp4, nsp7-8, nsp9, nsp10, and nsp11), or anti-FLAG monoclonal antibody (nsp5, nsp6, and nsp12). DyLight 549-conjugated goat anti-rabbit antibody was used as secondary antibody. (b) HEK293T cells were cotransfected with p125-Luc, pRL-SV40, and pCAGGS expressing nsps or pCAGGS empty vector for 24 h. Cells were harvested and measured for firefly and Renilla luciferase activities. Relative luciferase (RLU) activity is defined as a ratio of firefly luciferase reporter activity to Renilla luciferase activity. Each data point shown represents a mean value ± standard error of the mean (SEM) from three experiments.
