Leczyme: A New Candidate Drug for Cancer Therapy

Table 1

Inhibition of SBL-induced tumor cell agglutination by various compounds.

(a)

Mono- and oligosaccharides	Inhibitory effect
Xylose	−
D-Arabinose	−
L-Rhamnose	−
L-Fucose	−
D-Glucose	−
D-Galactose	−
D-Mannose	−
D-N-Acetylglucosamine	−
D-N-Acetylgalactosamine	−
Sucrose	−
Trehalose	−
Cellobiose	−
Melibiose	−
Lactose	−
Raffinose	−
N-Acetylneuraminyllactose	+

(b)

	Concentration inhibiting 100% of lectin activity
Glycoproteins	(𝜇g/200 𝜇L)
Mucin	3.2–6.4^a (30^b)
Asialomucin	70–140^b
Fetuin	125–250
Asialofetuin	600–1000
Transferrin	>250
Asialotransferrin	>250
Ovomucoid	>250
Glycosaminoglycans	(𝜇g/200 𝜇L)
Heparin	0.25–0.5
Chondroitin sulfate A	>300
Chondroitin sulfate B	17–35
Chondroitin sulfate C	>300
Keratan sulfate	250
Hyaluronic acid	>300
Polyamines	(mM)
Putrescine	25–50
Spermidine	12.5
Spermine	3.1

Data were summarized from [40, 42]. All evaluation of inhibiting effect was done against SBL (25 ng/200 𝜇L)-induced agglutination of AH109A cells. In mono- and oligosaccharides, inhibitory effect was assessed negative (−) or positive (+). All saccharides tested here do not inhibit the lectin activity of SBL even at 100 mM, except N-acetylneuraminyllactose, which shows weak inhibition effect at 50 mM. In other compounds, the concentration that inhibits 100% of the lectin activity was indicated [(𝜇g/200) for glycoproteins and glycosaminoglycans and (mM) for polyamines]. Note that inhibitory effects of mucin and fetuin are reduced by sialidase treatment of them. ^aBovine submaxillary mucin (type I) from Sigma. ^bBovine submaxillary mucin from Worthington Biochemical Co.