Inhibition of SBL-induced tumor cell agglutination by various compounds.
(a)
Mono- and oligosaccharides
Inhibitory effect
Xylose
−
D-Arabinose
−
L-Rhamnose
−
L-Fucose
−
D-Glucose
−
D-Galactose
−
D-Mannose
−
D-N-Acetylglucosamine
−
D-N-Acetylgalactosamine
−
Sucrose
−
Trehalose
−
Cellobiose
−
Melibiose
−
Lactose
−
Raffinose
−
N-Acetylneuraminyllactose
+
(b)
Concentration inhibiting 100% of lectin activity
Glycoproteins
(g/200 L)
Mucin
3.2–6.4a (30b)
Asialomucin
70–140b
Fetuin
125–250
Asialofetuin
600–1000
Transferrin
>250
Asialotransferrin
>250
Ovomucoid
>250
Glycosaminoglycans
(g/200 L)
Heparin
0.25–0.5
Chondroitin sulfate A
>300
Chondroitin sulfate B
17–35
Chondroitin sulfate C
>300
Keratan sulfate
250
Hyaluronic acid
>300
Polyamines
(mM)
Putrescine
25–50
Spermidine
12.5
Spermine
3.1
Data were summarized from [40, 42]. All evaluation of inhibiting effect was done against SBL (25 ng/200 L)-induced agglutination of AH109A cells. In mono- and oligosaccharides, inhibitory effect was assessed negative (−) or positive (+). All saccharides tested here do not inhibit the lectin activity of SBL even at 100 mM, except N-acetylneuraminyllactose, which shows weak inhibition effect at 50 mM. In other compounds, the concentration that inhibits 100% of the lectin activity was indicated (g/200) for glycoproteins and glycosaminoglycans and (mM) for polyamines. Note that inhibitory effects of mucin and fetuin are reduced by sialidase treatment of them. aBovine submaxillary mucin (type I) from Sigma. bBovine submaxillary mucin from Worthington Biochemical Co.