Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against Bordetella pertussis Infection
Figure 2
Pentamer formation of CTB-Fim2. (a) SDS-PAGE with purified CTB-Fim2. Lane 1 is the sample boiled in reducing conditions while lane 2 is the same protein sample not subjected to boiling and in nonreducing conditions. Bands of 36 and 180 kDa of CTB-Fim2 are shown, respectively. (b) GM1-ELISA to verify the ability of the pentamer to bind GM1-ganglioside using recombinant CTB-Fim2. Commercial CTB was used as a positive control. The 96-well plates were coated with GM1 or BSA and mouse anti-CTB antisera (1 : 2500).
