Comparison of a New Gold Immunochromatographic Assay for the Rapid Diagnosis of the Novel Influenza A (H7N9) Virus with Cell Culture and a Real-Time Reverse-Transcription PCR Assay
Table 4
The performance of GICA and RT-PCR assay with samples collected at different time points of infection.
Days after contact with poultry
Number of samples
RT-PCR assay
GICA
Sensitivita (%)
Specificitya (%)
Positive sample number (%)
Ct value median (range)
Positive sample number (%)
Ct value median (range)
0–7
11
7 (63.64%)
34.42 (22.77–39.16)
2 (18.18%)
29.37 (27.68–31.06)
28.57%
100.00%
8–10
23
17 (73.91%)
31.03 (16.68–37.87)
7 (30.43%)
26.39 (16.68–30.40)
41.18%
100.00%
11–14
45
36 (80.00%)
33.44 (23.10–36.48)
13 (28.89%)
27.72 (24.86–32.51)
36.11%
100.00%
15–21
42
29 (69.05%)
34.72 (19.15–45.08)
11 (26.19%)
29.04 (19.15–30.79)
34.48%
92.31%
>22
19
10 (52.63%)
36.20 (23.48–47.09)
1 (5.26%)
31.17
10.00%
100.00%
These parameters for GICA were obtained in reference to the RT-PCR assay.
