Differential Host Cell Gene Expression and Regulation of Cell Cycle Progression by Nonstructural Protein 11 of Porcine Reproductive and Respiratory Syndrome Virus

Figure 3

Confirmation of differential gene expression by RT-qPCR in MARC-nsp11 cells. The same preparations that were used for RNA microarray were used for RT-qPCR. For quantitative PCR, two genes (NOL6 and EGR1) were chosen to represent upregulated genes by nsp11, and TNFSF10 and DEPTOR were chosen as the downregulated genes in the microarray assays (Table 2). SH2 was chosen as a nonregulated gene. Bars illustrate the differential gene expression determined by RT-qPCR. White bars represent MARC-145 cells and black bars indicate MARC-nsp11 cells. Numbers in the table show the fold changes.