BioMed Research International

Research Article

Experimental Measurements for the Effect of Dilution Procedure in Blood Esterases as Animals Biomarker for Exposure to OP Compounds

Table 3

Kinetic parameters for hydrolysis of esterase in various dilutions.


Preincubation condition	CbE			ChE
Preincubation condition	(M)	(s⁻¹)	(s⁻¹ M⁻¹)	(M)	(s⁻¹)	(s⁻¹ M⁻¹)

Dilution (1 : 5)	176 ± 15.3^a	16,765 ± 865^a	95.3^a	265 ± 23.4^a	2023 ± 232^a	7.6^a
Dilution (1 : 10)	543 ± 21.3	12,765 ± 932	23.5	234 ± 32.4	17,165 ± 654	73.4
Dilution (1 : 15)	753 ± 28.4	7653 ± 121^b	10.2	943 ± 71.2	1154 ± 111	1.2

Values in the table are the mean ± SE of three individual serum esterase (CbE and ChE) for quail. For the measurement of and , esterase enzyme was inhibited for 30 min at room temperature 25°C with appropriate concentrations for malathion compound. Then results were fitted with an exponential decay using SigmaPlot 11. ^a,bdenotes significant difference (analysis of variance (ANOVA), ) between dilution 1 : 5 and other dilutions.