A Multiplex Snapback Primer System for the Enrichment and Detection of JAK2 V617F and MPL W515L/K Mutations in Philadelphia-Negative Myeloproliferative Neoplasms
Figure 2
Analytical sensitivity of the multiplex snapback primer system for mutant allele discrimination. (a) Serial diluted standards of 0.1% load MPL W515L (blue), MPL W515K (purple), and JAK2 V617F (ginger) specifically generated the corresponding melting peaks, which could be easily discriminated from the wild-type control (lake blue). (b) Serial JAK2 V617F dilution of 0.01% (pink), 0.1% (purple), 1% (blue), and 10% (ginger) mutation load. After the robust mutation enrichment, snapback primer system generated a melting curve with 0.1% mutation load that can be distinguished from the wild-type control.
