A Multiplex Snapback Primer System for the Enrichment and Detection of JAK2 V617F and MPL W515L/K Mutations in Philadelphia-Negative Myeloproliferative Neoplasms

Figure 4

ARMS for MPL W515L and MPL W515K mutation. The MPL W515L/K ARMS products after 2% agarose electrophoresis. The 246 bp band represented the amplification control. Bands of 98 bp were the wild-type allele product. Presence of the 188 bp amplicon suggested the mutant allele in DNA sample. MPL W515L ARMS system was in tracks of 1, 3, 5, 7, and 9; MPL W515K ARMS system was in tracks of 2, 4, 6, 8, and 10. M: 1,000 bp DNA ladder; BLK: no template amplification control; 1, 2: artificial plasmid with MPL W515L allele; 3, 4: artificial plasmid with MPL W515K allele; 5, 6: RPMI 8226 cell line DNA; 7, 8: patient sample with heterozygous MPL W515L mutation; 9, 10: patient sample with heterozygous MPL W515K mutation.