Biological Evaluation of 131I- and CF750-Labeled Dmab(scFv)-Fc Antibodies for Xenograft Imaging of CD25-Positive Tumors
Figure 2
Binding specificity of the Dmab(scFv)-Fc antibody. (a) CD25-negative SMMC7721 cells and CD25-positive Hut102 cells were incubated with FITC-labeled Dmab(scFv)-Fc antibody or Dmab(scFv) antibody, followed by flow cytometric analysis. (b) Hut102 tumor tissues and liver tissues were stained with FITC-labeled Dmab(scFv)-Fc antibody and observed under a fluorescence microscope. DAPI was used to visualize the cell nucleus. An isotype antibody was used as a control.
