Biological Evaluation of ¹³¹I- and CF750-Labeled Dmab(scFv)-Fc Antibodies for Xenograft Imaging of CD25-Positive Tumors

Figure 4

Immunoreactivity of ¹³¹I-Dmab(scFv)-Fc antibody and SPECT/CT of mice bearing Hut102 tumor xenografts at 5 h after injection. (a) Immunoreactivity of ¹³¹I-labeled Dmab(scFv)-Fc antibody was analyzed using Hut102 cell binding assays. The same amount of free ¹³¹I was used as a control. (b) After intravenous injection of ¹³¹I-labeled Dmab(scFv)-Fc antibody (185 kBq/g body weight), the mice were scanned by using a Precedence 6 slice SPECT/CT machine. Different angles of the tumor graft (arrow) are displayed, including a sectional view (b-1), normal view (b-2), and side view (b-3).

(a)

(b)