Punicalagin and Ellagic Acid Demonstrate Antimutagenic Activity and Inhibition of Benzo[a]pyrene Induced DNA Adducts

Table 8

Effect of ellagic acid on the methyl methanesulfonate induced mutagenicity in Salmonella typhimurium.

Treatment	Dose (μM)	Number of His^+ revertants colonies/plate (mean ± SE)
		TA 97a	TA 98	TA 100	TA 102
Spontaneous		142.0±5.3	33.7±2.0	128.3±4.9	240.7±7.0
Positive control (MMS)	1.5 μg	449.7±7.2	53.7±1.5	963.0±11.4	1237.0±14.5
^aEllagic acid	50	189.7±6.4	50.7±1.5	179.7±9.1	338.0±15.6
	100	163.0±5.2	38.0±1.7	170.0±5.7	311.0±12.1
	250	145.3±4.3	34.3±2.4	155.7±10.3	274.0±7.6
	500	134.0±3.8	30.0±1.2	132.3±8.6	228.0±11.0
^bEllagic acid + MMS	50	426.3±11.6 (9.0)	53.0±1.2 (22.2)	900.3±16.3^∗ (8.0)	1071.0±16.7^∗∗ (18.5)
	100	378.0±10.8^∗∗ (25.0)	48.3±2.0 (34.0)	754.0±8.2^∗∗∗ (26.4)	956.0±19.6^∗∗∗ (30.3)
	250	321.3±10.8^∗∗∗ (42.2)	44.0±2.1^∗ (50.0)	598.7±14.2^∗∗∗ (45.1)	810.0±19.1^∗∗∗ (44.3)
	500	239.7±10.8^∗∗∗ (66.5)	37.3±2.6^∗∗ (69.0)	420.3±6.9^∗∗∗ (65.3)	506.0±13.5^∗∗∗ (72.4)
𝑅^2		0.98	0.99	0.99	0.95

aNegative control; ^bpreincubation test; values in parenthesis are % inhibition of mutagenicity.
^∗𝑃<0.05; ^∗∗𝑃<0.005 and ^∗∗∗𝑃<0.001; MMS: methyl methanesulfonate; 𝑅²: linear regression analysis.