Research Article

Molecular Characterization of a Recombinant Manganese Superoxide Dismutase from Lactococcus lactis M4

Figure 2

Analysis of denatured protein on 12% SDS-PAGE (a) and detection of recombinant SOD on western blotting PVDF membrane (b). Lane 1(a): protein molecular weight marker; lane 1(b): prestained protein marker (NEB); lane 2: crude extract of L. lactis M4; lane 3: pRSET/SOD; lane 4: pRSET A; lane 5: E. coli BL21(DE3)pLysS were with the addition of 1 mm IPTG; lane 6: pRSET/SOD; lane 7: pRSET A; lane 8: E. coli BL21(DE3)pLysS were without the addition of 1 mM IPTG. Arrows indicate the presence of the expressed SOD at about 27 kDa after staining using Coomassie Brilliant Blue staining for SDS-PAGE and detecting of horseradish peroxidase (HRP) conjugated secondary antibody with 3,3′-diaminobenzidine (DAB). The intensity of the recombinant SOD protein band with IPTG induction is significantly higher than the noninduced recombinant SOD.
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469298.fig.002b
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