Molecular Characterization of a Recombinant Manganese Superoxide Dismutase from Lactococcus lactis M4

Table 1

Summary of the purification procedure for the SOD from Lactococcus lactis M4.

	Total activity (U)	Total protein (mg)	Specific activity (U/mg)	Yield (%)	Purification fold
Crude extract	16,982	88.9	4,967	100.00	1.00
IMAC	7,504	12.5	8,706	44.19	1.75
Gel filtration	3,796	7.1	18,076	22.35	3.64
Dialysis	3,879	6.9	18,650	22.84	3.75

Note: IMAC represents immobilized metal affinity chromatography. Two peaks of active SOD activity were detected after IMAC. Only peak 2 was subjected to further purification steps.