Pinocembrin Protects Human Brain Microvascular Endothelial Cells against Fibrillar Amyloid- β₁₋₄₀ Injury by Suppressing the MAPK/NF- κ B Inflammatory Pathways

Figure 4

Effects of pinocembrin on MAPK pathways of hBMECs against fAβ _1–40-induced toxicity. (a) Images of phosphor-p38, phosphor-MK2, phosphor-SAPK/JNK, phosphor-c-Jun, and phosphor-ERK1/2 were acquired on the ArrayScan HCS Reader using the Cytoplasm to Nucleus Translocation BioApplication (×20). (b), (c), and (e) Values of Mean_CircRingAvgIntenDiff describe the capacity translocation of cytosolic phospho-p38, phospho-SAPK/JNK, and phospho-ERK1/2 to the nucleus and nuclear phospho-MK2 to the cytoplasm. (d) Nuclear average fluorescence intensity illustrates the expression of phosphor-c-Jun. Data are expressed as means ± SEM, 𝑛=6, ^∗∗∗𝑃<0.001 versus control, ^#𝑃<0.05, ^##𝑃<0.01, ^###𝑃<0.001 versus fAβ _1–40.

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