The Putative HORMA Domain Protein Atg101 Dimerizes and Is Required for Starvation-Induced and Selective Autophagy in Drosophila
Figure 10
FLAG-tagged ref(2)P coprecipitates with both HA-Atg101 and HA-Atg8a, but not with anti-HA beads (a). Note that HA-Atg8a-II migrates faster than HA-Atg8a-I, due to the covalent attachment of the lipid moiety phosphatidylethanolamine to its C-terminus. (b) The selective autophagy cargo ref(2)P coprecipitates with Atg8a, Atg101, Atg18, RagC, Atg13, Atg16, and Keap1, but not with empty beads.
