Cell Type-Dependent RNA Recombination Frequency in the Japanese Encephalitis Virus

Figure 1

The schematic sketch designed to identify RNA recombination between viral strains. A fragment (868 bp) comprised of the C/preM junction (nt 10~877) of viral RNA extracted from coinfected BHK-21 or C6/36 cells was amplified, cloned, and then used for an RFLP analysis with SmaI or Alw44I. Two and one recombinant form(s) were, respectively, identified in selected samples from BHK-21 and C6/36 cells, when they were coinfected with the T1P1-S1 and CJN-S1 strains of the Japanese encephalitis virus.