Cell Type-Dependent RNA Recombination Frequency in the Japanese Encephalitis Virus
Figure 3
Status of RNA recombination after inhibition by exoribonuclease with PAP (3′-phosphoadenosine-5′-phosphate, an inhibitor of exoribonuclease). (a) The RNA recombination rate increased to a higher level in BHK-21 cells after treatment with PAP, compared to that of untreated cells. In contrast, no effect of PAP on increasing RNA recombination of the virus was shown in C6/36 cells despite a very low level of RNA recombination still being observed. Viral RNA was not affected after treatment with PAP, suggesting exoribonuclease-mediated degradation of transfected RNA fragments might increase RNA recombination of the virus strains, especially in mammalian cells. (b) Treatment with PAP in C6/36 cells did not cause degradation of the transfected (+) RNA fragment at 3 h until 6 h after transfection at which a partial effect appeared.
