Cell Type-Dependent RNA Recombination Frequency in the Japanese Encephalitis Virus

Figure 4

Degradation of double-stranded (ds) RNA fragments transfected into mosquito cells. A fragment (807 bp) of viral RNA extracted from C6/36 cells was detected through an RT-PCR at 0 h after transfection (hpt) with dsRNA derived from (+) or (−) 5′3′-UTR RNA. The transfected dsRNA had faded at 3 and 6 h after transfection, suggesting that dsRNAs may have been cleaved, and thus generated undetectable short interfering RNAs.