Cell Type-Dependent RNA Recombination Frequency in the Japanese Encephalitis Virus
Figure 5
Viral RNA, either T1P1-S1 or CJN-S1, accumulated in C6/36 cells more slowly than in BHK-21 cells. (a) The RNA amount of T1P1-S1 remained at the baseline level until 12 h after infection (hpi) (3.81-fold change), which increased to 169.72-fold at 24 hpi in C6/36 cells. In contrast, T1P1-S1 RNA, respectively, increased to 3.09-, 28.99-, 429.05-, 4396.07-, and 5487.75-fold, at 3, 6, 9, 12, and 24 hpi in BHK-21 cells. The RNA amount of CJN-S1 also accumulated more slowly in C6/36 cells than BHK-21 cells, which remained at the baseline level until 12 hpi (2.36-fold increase) and had increased to 152.32-fold by 24 hpi in C6/36 cells. Although the amount of CJN-S1 RNA did not evidently increase until 6 hpi (1.35-fold change), it increased to 16.64-, 111.43-, and 554.87-fold at 9, 12, and 24 hpi, respectively, in BHK-21 cells. (b) Stability of viral RNA was evaluated after a fragment of (+)5′3′-UTR-II RNA was transfected into BHK-21 or C6/36 cells. Transfected fragments were insignificantly degraded even at 3 or 6 h after transfection in BHK-21 cells while more obvious degradation appeared in C6/36 cells.
