Cell Type-Dependent RNA Recombination Frequency in the Japanese Encephalitis Virus

Table 1

Identification of RNA recombination of the Japanese encephalitis virus based on a fragment (868 bp) comprised of the C/preM junction (nt 10~877) of viral RNA extracted from coinfected BHK-21 or C6/36 cells using an RFLP analysis with restriction enzymes SmaI or Alw44I.

Treatment	BHK-21 cells		C6/36 cells
	Number of detection	Number of recombination	Number of detection	Number of recombination
Coinfected viral genomic RNA	98	20 (20.4%)	38	5 (13.1%)
Mixed RNA*	44	2 (4.5%)	39	3 (7.7%)
Stastistical analysis**	𝑃<0.05		𝑃>0.05

∗Mixed RNA was a mixture of RNAs separately extracted from T1P1-S1 and CJN-S1 strains of the Japanese encephalitis virus, being used as the internal control.
∗∗Yates’ chi-square test was used to assess the difference of RNA recombination in cells coinfected by two virus strains at 5% level of significance.