Fatty Acids in Energy Metabolism of the Central Nervous System

Figure 7

Respiratory activity and membrane potential of the rat (Lewis) brain mitochondria isolated in the absence (a) and in the presence (b) of 0.1% BSA. Incubation conditions: 125 mM KCl, 10 mM NaCl, 10 mM MOPS, pH 7.2, 2 mM MgCl₂, 2 mM KH₂PO₄, 1mM EGTA, and 0.7 mM CaCl₂. At Ca²⁺/EGTA = 0.7, the [Ca²⁺]_Free was 1 µM. Chamber volume = 0.65 mL. Substrate: succinate 5 mM, no rotenone was added. Additions: BM 0.3 mg, ADP 150 μM, and CCCP 0.5 μM. Numbers at the traces are respiratory activities in nmol/min/mg mitochondrial protein. Respiratory activity ratio (RCR) is 𝑉_State3/𝑉_State4.

(a)

(b)