Peripheral Injection of SB203580 Inhibits the Inflammatory-Dependent Synthesis of Proinflammatory Cytokines in the Hypothalamus

Table 1

All genes analyzed by real-time PCR are listed with their full name and abbreviation.

GenBank acc.; number	Gene	Amplicon size [bp]	Forward/reverse	Sequence 5′→3′
NM_001034034	GAPDH; Glyceraldehyde-3-phosphate dehydrogenase	134	Forward; Reverse	AGAAGGCTGGGGCTCACT; GGCATTGCTGACAATCTTGA
U39357	ACTB; Beta actin	168	Forward; Reverse	CTTCCTTCCTGGGCATGG; GGGCAGTGATCTCTTTCTGC
NM_001076910	PPIC; Cyclophilin C	131	Forward; Reverse	ACGGCCAAGGTCTTCTTTG; TATCCTTTCTCTCCCGTTGC
BC108088.1	HDC1; Bos taurus histone deacetylase 1	115	Forward; Reverse	CTGGGGACCTACGGGATATT; GACATGACCGGCTTGAAAAT
X54796.1	IL-1 𝜷; Interleukin-1 beta	137	Forward; Reverse	CAGCCGTGCAGTCAGTAAAA; GAAGCTCATGCAGAACACCA
NM_001206735.1	IL-1R1; Interleukin-1 receptor, type I	124	Forward; Reverse	GGGAAGGGTCCACCTGTAAC; ACAATGCTTTCCCCAACGTA
NM_001009392.1	IL-6; Interleukin-6	165	Forward; Reverse	GTTCAATCAGGCGATTTGCT; CCTGCGATCTTTTCCTTCAG
NM_001110785	IL-6R; Interleukin 6 receptor	149	Forward; Reverse	TCAGCGACTCCGGAAACTAT; CCGAGGACTCCACTCACAAT
NM_001024860	TNF 𝜶; Tumor necrosis factor alpha	153	Forward; Reverse	CAAATAACAAGCCGGTAGCC; AGATGAGGTAAAGCCCGTCA
NM_174674	TNFR1; Tumor necrosis factor receptor superfamily, member 1A	137	Forward; Reverse	AGGTGCCGGGATGAAATGTT; CAGAGGCTGCAGTTCAGACA
NM_001040490	TNFR2; Tumor necrosis factor receptor superfamily, member 1B	122	Forward; Reverse	ACCTTCTTCCTCCTCCCAAA; AGAAGCAGACCCAATGCTGT