[Retracted] Leaf Rolling and Stem Fasciation in Grass Pea (Lathyrus sativus L.) Mutant Are Mediated through Glutathione-Dependent Cellular and Metabolic Changes and Associated with a Metabolic Diversion through Cysteine during Phenotypic Reversal

Figure 10

Representative imaging of superoxide radicals and H₂O₂ productions in Lathyrus sativus L. leaves by CLSM. Images are developed from several optical sections collected by confocal microscopy showing the autofluorescence (blue; excitation at 633 nm, emission at 680 nm) and fluorescence due to DHE and DCF-DA. Superoxide-dependent DHE fluorescence (red) in leaf cross sections from (a) mother control, (b) mutant control, (c) BSO-treated mutant, (d) BSO-treated mother with blue autofluorescence, and (e) stem cross section from BSO-treated mutant; ((f), (g)) for the negative control, leaves (f) and stems (g) of mutant were incubated with 1 mmTMP, a superoxide scavenger; ((h)–(n)) H₂O₂-dependent DCF-DA fluorescence (green) in leaf cross sections of (h) mother control, (i) mutant control, (j) BSO-treated mother, (k) BSO-treated mutant, and (l) stem cross section of BSO-treated mutant; ((m), (n)) as a negative control, leaves were incubated with 1 mm ascorbate (ASC), which acts as a H₂O₂ scavenger in BSO-treated (m) mother and (n) treated mutant, (o) stem cross section of stem and (p) leaves of thiourea + BSO-treated mutant with DHE fluorescence (red), (q) thiourea + BSO-treated leaves, and (r) stems of mutant with H₂O₂-dependent DCF-DA fluorescence (green). The results are representative of at least three independent experiments. Ep: epidermis; Hp: hypodermis; Ms: mesophyll cells; Scl: sclerenchyma; Xy: xylem vessels. Bars = 50 𝜇m.

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