Extracts from Vatica diospyroides Type SS Fruit Show Low Dose Activity against MDA-MB-468 Breast Cancer Cell-Line via Apoptotic Action

Table 2

The acetone and methanol extracts of V. diospyroides type SS fruit at their IC₅₀ concentrations (see Table 1) were inactive on normal Vero cells. The cell growth was assessed by a green fluorescent protein (GFP) assay.

Extract-fruit part	Extract concentrations (μg/mL)	% Cell growth*	Activity	IC50 (μg/mL)
Acetone-cotyledon	50.00	26.53 ± 11.34^b	Cytotoxic	43.90 ± 4.67
	16.67	102.74 ± 2.56^a	Noncytotoxic	N/A
	5.56	104.17 ± 14.26^a	Noncytotoxic	N/A
	1.85	108.71 ± 10.00^a	Noncytotoxic	N/A
	0.62	107.94 ± 10.79^a	Noncytotoxic	N/A
	0.21	110.78 ± 12.40^a	Noncytotoxic	N/A
Acetone-pericarp	50.00	103.49 ± 32.03^a	Noncytotoxic	N/A
	16.67	114.56 ± 7.96^a	Noncytotoxic	N/A
	5.56	110.46 ± 11.19^a	Noncytotoxic	N/A
	1.85	108.13 ± 10.80^a	Noncytotoxic	N/A
	0.62	110.54 ± 5.94^a	Noncytotoxic	N/A
	0.21	111.26 ± 6.65^a	Noncytotoxic	N/A
Methanol-cotyledon	50.00	88.01 ± 15.42^a	Noncytotoxic	N/A
	16.67	115.46 ± 11.87^a	Noncytotoxic	N/A
	5.56	112.69 ± 8.84^a	Noncytotoxic	N/A
	1.85	116.70 ± 11.63^a	Noncytotoxic	N/A
	0.62	116.26 ± 11.07^a	Noncytotoxic	N/A
	0.21	117.57 ± 7.79^a	Noncytotoxic	N/A
Methanol-pericarp	50.00	25.34 ± 15.50^b	Cytotoxic	48.75 ± 0.79
	16.67	112.68 ± 6.99^a	Noncytotoxic	N/A
	5.56	104.09 ± 6.55^a	Noncytotoxic	N/A
	1.85	104.45 ± 11.47^a	Noncytotoxic	N/A
	0.62	104.64 ± 6.67^a	Noncytotoxic	N/A
	0.21	104.89 ± 9.67^a	Noncytotoxic	N/A

N/A means not applicable IC₅₀ calculation.
-*Cell growth reduced to less than 50% would indicate cytotoxicity against the Vero cells; otherwise an extract was considered inactive and the IC₅₀ level could not be determined (N/A). Three independent replicates were performed.
-Different superscripts within a column indicate statistically significant differences in the Vero cell growth (mean ± SD) according to Tukey’s multiple comparison test.