BioMed Research International

Research Article

Chitinase from a Novel Strain of Serratia marcescens JPP1 for Biocontrol of Aflatoxin: Molecular Characterization and Production Optimization Using Response Surface Methodology

Table 1

Experimental design and results of CCD of 4 variables in coded and actual units.
Runs : glucose
(g/L)		 : peptone
(g/L)		 : chitin
(g/L)		 : (NH4)2SO4 
(g/L)		Chitinase activity (U/mL)
CodedConc.CodedConc.CodedConc.CodedConc.ObservedPredicted
1−14−12−18−12 7.56
218−12−18−12 9.24
3−1414−18−12 16.11
41814−18−12 18.08
5−14−12−1814 8.75
618−12−1814 9.87
7−1414−1814 17.45
81814−1814 18.87
9−14−12112−12 5.05
1018−12112−12 7.03
11−1414112−12 12.89
121814112−12 15.17
13−14−1211214 5.57
1418−1211214 7.00
15−141411214 13.57
16181411214 15.29
17−220301003 9.51
182100301003 12.91
1906−2101003 4.05
20062501003 20.89
210603010−21 11.72
22060301025 13.03
230603−2603 13.86
24060321403 7.76
25060301003 13.23
26060301003 13.23
27060301003 13.23
28060301003 13.23
29060301003 13.23
30060301003 13.23
31060301003 13.23