Research Article

Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris

Figure 4

CvHSP90 mRNA expression levels under different heat shock temperatures (5°C, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C, 40°C, and 45°C) for 1 h were analyzed by real-time quantitative reverse transcriptase-polymerase chain reaction. The β-actin gene was used as an internal control to calibrate the cDNA template for all the samples. Vertical bars represented the mean ± SE ( ).
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