Research Article
Cloning and Expression of a Cytosolic HSP90 Gene in Chlorella vulgaris
Table 1
PCR primers used in this study.
| Primers | Sequence (5′-3′) | Sequence information |
| P1 | GNGTGTTCATCATGGACAAYTGYGA | Homology cloning primer | P2 | TTCATGATGCKYTCCATGTTNGC | Homology cloning primer | P3 | GCCCCTCAACATCTCCCGCG | 3′-RACE primer | P4 | ACCTGCCCCTCAACATCTCCC | 3′-RACE primer | adaptor | GGCACGCGTCGACTAGTAC | 3′-RACE primer | P5 | TTTTGTCCTCCTCGGTCTCG | 5′-RACE primer | P6 | GCCCTCCTTGGTCACGCTCA | 5′-RACE primer | Oligo(dG)-adaptor | GGCACGCGTCGACTAGTACG10 | 5′-RACE primer | P7 | GGCCAGTTCGGCGTGGGTTT | Real time gene primer | P8 | AAGCGACGGGTTGACGGTGT | Real time gene primer | P9 | AACTACGAGCTGCCAGACGG | Real time actin primer | P10 | GAAGACAAGACGATGCTGAGGG | Real time actin primer | M13-47 | CGCCAGGGTTTTCCAGTCACGAC | Sequencing primer | RV-M | GAGCGGATAACAATTTCACACAGG | Sequencing primer |
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