Evaluation of Two Different Analytical Methods for Circulating Tumor Cell Detection in Peripheral Blood of Patients with Primary Breast Cancer
Figure 2
(a) shows images with CTC and artifacts from one patient presented in the gallery of the CSS. CTC show the characteristics defined before: round or oval shape, positive signal in the cytokeratin channel (second column), intact nucleus (third column), overlapping of nucleus and cytokeratin signal (first column), as well as no signal for CD45 as leucocytes marker (column 4), and in the negative control channel (fifth column). In comparison (b) shows a sample of a CTC detected by the MICC. Cells which are labeled with the anti-cytokeratin-antibody A45-B/B3 are then detected by the Z0259 antibody using the APAAP method and appear bright red.
