Comprehensive Transcriptome Study to Develop Molecular Resources of the Copepod Calanus sinicus for Their Potential Ecological Applications

Figure 6

Melting curve of locus CsSNP02 genotyped with 𝑇_𝑚-shift method. GC tails of different lengths were added to allele-specific primers. Samples homozygous for allele A or T will be amplified with the short GC-tailed primer and show lower temperature peak. Samples homozygous for allele G or C will be amplified with the long GC-tailed primer and show higher temperature peak. Samples heterozygous will show both temperature peaks.