Mechanical Stress Stimulates the Osteo/Odontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 1/2 and JNK MAPK Pathways

Figure 4

Real-time reverse transcription-polymerase chain reaction and Western blot analyses of SCAPs in different groups. (a) Gene expressions of RUNX2, OSX, OCN, and DSPP after 0-day, 3-day, and 7-day cultures. GAPDH was used as an internal control for each group. Gene expression was described as a fold change relative to the control group. Values are described as the mean±SD, 𝑛=3. (^∗∗2^−ΔΔCt≥2, 𝑃<0.01; ^∗1<2^−ΔΔCt<2, 𝑃<0.01). (b) Protein expressions of RUNX2, OSX, and OCN after 0-day, 3-day, and 7-day cultures. β-actin was used as a control for each group.

(a)

(b)