L-Methionase: A Therapeutic Enzyme to Treat Malignancies

Table 3

Molecular cloning and functional characterization of MGL gene in various expression systems.

Gene from	Host strain for expression	Selectable marker	References
P. putida.	E. coli MV1184	Ampicillin	[35]
P. putida	E. coli BL21 (DE3)	Ampicillin	[36]
P. putida ICR3460	E. coli JM109	Tetracycline	[37]
P. putida	Lung cancer cell line H460	GFP fluorescence, Penicillin, Streptomycin	[38]
P. putida	Human lung adenocarcinoma epithelial cell line (A549 cells)	G418 (Geneticin)	[39]
P. putida ICR3460	E. coli JM109	Tetracycline	[40]
P. putida ICR3460	E. coli JM109	Tetracycline	[41]
Trichomonas vaginalis	E.  coli M15pREP4	Ampicillin and Kanamycin	[42]
Trichomonas vaginalis	E.  coli M15[pREP4]	Ampicillin and Kanamycin	[43]
Treponema denticola ATCC35405	E. coli BL21	Ampicillin	[19]
Entamoeba histolytica	E. coli BL21	Ampicillin	[22]
Kluyveromyces lactis CLIB 640	E. coli DH10B	Ampicillin	[44]
Arabidopsis	E. coli BL21	Carbenicillin	[24]
Brevibacterium linens	E. coli DH5α	Ampicillin	[45]