Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus

Figure 2

Sensitivity of H7-RT-LAMP and N9-RT-LAMP for the detection of H7N9 influenza viruses that were isolated from various sources. ((a) to (d)) A/chicken/Shanghai/S1053/2013 (H7N9), A/pigeon/Shanghai/S1069/2013(H7N9), A/environment/Shanghai/S1088/2013 (H7N9), and A/Anhui/1/2013 (H7N9) RNAs were amplified using H7-RT-LAMP. ((e) to (h)) A/chicken/Shanghai/S1053/2013 (H7N9), A/pigeon/Shanghai/S1069/2013 (H7N9), A/environment/Shanghai/S1088/2013 (H7N9), and A/Anhui/1/2013 (H7N9) RNAs were amplified using N9-RT-LAMP. Viral RNA concentrations ranging from 10,000 to 0.001 PFU per tube were tested. LAMP products were detected by a real-time turbidity assay using an LA-320c.