Development of a Reverse Transcription Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Subtype H7N9 Avian Influenza Virus
Figure 3
Comparative sensitivity of the RT-LAMP and RT-PCR methods. N9-RT LAMP and RT-PCR were performed using A/Shanghai/4664T/2013 (H7N9) viral RNA at concentrations ranging from 10,000 to 0.001 PFU per tube. ((a) and (b)) Detection limit of N9-RT-LAMP. LAMP products were detected using a real-time turbidity assay with an LA-320c (a) and a fluorescence assay (b). (c) Detection limit for the one-step RT-PCR using the same RNA extracts that were used for N9-RT LAMP. The PCR products were observed in a 1.5% agarose gel that was stained with ethidium bromide.
