The Cytotoxicity of Benzaldehyde Nitrogen Mustard-2-Pyridine Carboxylic Acid Hydrazone Being Involved in Topoisomerase IIα Inhibition
Figure 4
DNA alkylation of BNMPH. (a) BNMPH effect on DNA thermal denaturation. The flatted curve (black) indicated that the dissociation of double stranded DNA was blocked during temperature rising due to cross-linking. (b) BNMPH induced DNA cross-linking. The cross-linked DNA was separated by electrophoresis and visualized by EB staining, and the concentrations used are as indicated.
