The Cytotoxicity of Benzaldehyde Nitrogen Mustard-2-Pyridine Carboxylic Acid Hydrazone Being Involved in Topoisomerase IIα Inhibition
Figure 8
BNMPH reactivity with DTT and BSA. (a) The absorbance spectral changes of DTT after addition of 26 μM BNMPH to 500 μM DTT (pH 8.0, 37°C). The spectrum was collected after the mixing, and subsequent spectrum at 5 min intervals was recorded. The insert showed a series of increases during the 25 min period, indicating that BNMPH can alkylate thiol group. (b) Spectrum of BNMPH (13 μM) reacted with thiol of BSA (5 μM) in pH 8.0 buffer at 37°C; the insert showed no obvious changes during the 25 min period, indicating that BNMPH may react with thiol group of BSA at very low rate.
