RNA-Seq Identifies Key Reproductive Gene Expression Alterations in Response to Cadmium Exposure

Table 1

Primers used for qPCR validation.

Gene	Primer sequence (5′-3′)	Target size (bp)	Tm (°C)
Actin, beta	Forward: CTGTCGAGTCGCGTCCACCC; Reverse: ACATGCCGGAGCCGTTGTCG	128	59
Cyp11a1	Forward: AGATCCCTTCCCCTGGTGACAATG; Reverse: CGCATGAGAAGAGTATCGACGCATC	192	60
Cyp17a1	Forward: CCAGGACCCAAGTGTGTTCT; Reverse: CCTGATACGAAGCACTTCTCG	250	59
Prm2	Forward: CAAGAGGCGTCGGTCA; Reverse: TGGCTCCAGGCAGAATG	167	59
Tex15	Forward: ATTTGAGTGGCACAGAC; Reverse: AGTATTGGGATTTGGAG	194	59
Adam9	Forward: CGCTTAGCAAACTACCTG; Reverse: TTCCCGCCACTGAACAA	147	59
Dazl	Forward: GGAGGCCAGCACTCAGTCTTC; Reverse: AGCCCTTCGACACACCAGTTC	184	60