Investigation of NF-κB1 and NF-κBIA Gene Polymorphism in Non-Small Cell Lung Cancer

Table 1

PCR and RFLP procedures and products of NF-κB1 -94ins/delATTG and NF-κBIA 3′UTR A→G.

	Primers (forward and reverse)	PCR product	Restriction enzyme	Restriction products
NF-κB1	5′-TGGGCACAAGTCGTTTATGA-3′	285 bp	Van91l (PflMI)	ATTG2/ATTG2 (ins/ins): 240 bp, 45 bp
	5′-CTGGAGCCGGTAGGGAAG-3′			ATTG1/ATTG1 (del/del): 281
				ATTG2/ATTG1 (ins/del): 281, 240, 45
NF-κBIA	5′-GGCTGAAAGAACATGGACTTG-3′	424 bp	HaeIII	AA wild type: 424
	5′-GTACACCATTTACAGGAGGG-3′			AG heterozygous: 316, 108
				GG mutant: 424, 316, 108