Development of a Colloidal Gold Kit for the Diagnosis of Severe Fever with Thrombocytopenia Syndrome Virus Infection

Figure 1

(a) A schematic representation of the immunochromatographic assay; the NSI-strip included five components: three pads (sample, conjugate, and absorbent), an NC membrane, and a plastic backing plate. The conjugate pad contained dried nanogold-Ab probe, which provided an easily visible color owing to the red color itself. There were three lines on the NC membrane: the control line and test lines (G and M). The control line contained Biotin-BSA; the G test zone included anti-human IgG antibody and the M test zone included anti-human IgM. (b) The result of negative detection, (c) the result of IgG positive detection, (d) the result of IgM positive detection, and (e) the result of both IgG and IgM positive detection.